Diagnostics

Effective management of infectious disease depends on both the early stage diagnosis and treatment of patients. For a number of infectious diseases, in addition to the lack of effective therapeutic treatments, there also exists a lack of adequate point-of-care diagnostics.

Arisan is collaborating with Professor Ahmet Ali Yanik at the University of California at Santa Cruz to develop novel, rapid nano-sensor diagnostics to facilitate early stage detection of infectious diseases. These diagnostics utilize nano-hole arrays engineered for incident light transmission through a novel process incorporating surface plasmon polaritons (SPPs), which allows efficient light transmission, commonly known as Extraordinary Light Transmission (EOT) only at designed resonance wavelengths. When antibodies (specific to a particular infectious agent) attached to a nano-hole chip array bind the infectious agent the wavelength of emitted light shifts to confirm detection of the infectious agent.

Illustrations depict (a) pre- and (b) post- incubation of patient blood samples on a nano-hole array chip containing antibodies to a given specific infectious agent. EOT spectra (c) before and (d) after sample incubation are shown. EOT resonance wavelengths red-shift only for those nanohole arrays for which infectious agents from the patient sample bind to agent-specific antibodies immobilized on the chip: Seeing protein monolayers with naked eye through plasmonic Fano resonances, Yanik, et al, Proceedings of the National Academy of Sciences (2011), Vol 108, pp. 11784-11789.

This collaborative program is supported by NIAID (GRANT R41AI127055).

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